초음파에너지를 이용한 칡으로부터 이소플라보노이드의 추출공정 최적화

이승범; 김수인; 홍인권; Seung Bum Lee; Su In Kim; In Kwon Hong

Applied Chemistry for Engineering, Vol.29, No.5, 503-509, October, 2018

DOI10.14478/ace.2018.1039 Export Citation

초음파에너지를 이용한 칡으로부터 이소플라보노이드의 추출공정 최적화

Optimization of Iso-flavonoids Extraction Process from Kudge Using Ultrasonic Irradiation Energy

이승범 , 김수인, 홍인권 ^†

단국대학교 화학공학과

Seung Bum Lee , Su In Kim, and In Kwon Hong^†

Department of Chemical Engineering, Dankook University, Gyeonggi 16890, Korea

E-mail:

초록

본 연구에서는 칡으로부터 항산화 물질을 추출하는 방법으로 초음파 추출공정을 이용하였으며, 칡에 포함된 iso-flavonoids인 puerarin, daidzein, daidzin의 함량을 측정하였다. 본 연구에서는 칡으로부터 iso-flavonoids 추출수율의 최대화를 위한 공정조건의 최적화를 위해 통계학적 분석방법인 반응표면분석법을 적용하였다. 이는 최소한의 실험횟수로 원하는 반응치에 부합하는 공정조건을 효과적으로 도출하고 각 공정조건이 반응치에 미치는 효과도를 분석하는 방법이다. 반응표면분석법 중 중심합성계획모델을 적용하여 최적조건을 분석하였으며, 3개의 독립변수는 초음파 조사시간, 주정/초순수 부피비, 초음파 조사세기로 설정하였다. 반응표면분석법을 이용하여 두 개의 반응치인 추출수율과 iso-flavonoids 함량의 최대값을 갖는 최적조건을 평가한 결과 초음파 조사시간(24.75 min), 주정/초순수 부피비(39.75 vol%), 초음파 조사세기(592.36 W)로 나타났다. 종합 만족도 D는 0.8938로 높게 나타났으며 5% 이내의 유의성 수준에 인정된다. 또한 최적화 과정을 분석한 결과 초음파 조사시간이 반응치에 가장 영향을 많이 미치는 인자임을 확인할 수 있었다.

In this study, we used the ultrasonic extraction process as a method to extract antioxidant substances from kudzu, and measured the content of iso-flavonoids puerarin, daidzein, daidzin contained in kudzu. The response surface methodology which is a statistical analysis method for optimizing the extraction amount of iso-flavonoids from the kudzu and the process condition for maximizing the yield was applied. It is the final objective of this study to effectively derive the condition of the process that matches the target response with a minimum number of experiments and analyze the effect of each process condition on the response. In the response surface methodology, the central composite design was applied and the optimum condition was analyzed, and the three independent variables were set to ultrasonic irradiation time, volume ratio of ethanol/ultrapure water, ultrasonic irradiation power. Using the response surface methodology, the optimum conditions with the maximum extraction yield and the content of iso-flavonoids were evaluated as ultrasonic irradiation time (24.75 min), ethanol / ultrapure water volume ratio (39.75 vol%), ultrasonic irradiation power (592.36 W). The overall satisfaction level appears as high as 0.8938, which is recognized at a significance level within 5%. As a result of analyzing the optimization process, it was confirmed that the ultrasonic irradiation time is the factor that most affects the responses.

Keywords:kudzu;iso-flavonoids;ultrasonic irradiation energy;response surface methodology

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Ameer K, Bae SW, Jo Y, Lee HG, Ameer A, Kwon JH, Food Chem., 229, 198 (2017)
Sharma J, Sukriti, Anand P, Pruthi V, Chaddha AS, Bhatia J, Kaith BS, Mater. Chem. Phys., 196, 270 (2017)
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[2] Choi SW, Kim KS, Hur NY, Ahn SC, Park CS, Kim BY, Baik MY, Kim CO, J. Life Sci., 18, 1447 (2008)

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