Microvalve-controlled miniaturized electrochemical lab-on-a-chip based biosensor for the detection of β-amyloid biomarker

Satish Kasturi; Sri Ramulu Torati; Yunji Eom; CheolGi Kim

Journal of Industrial and Engineering Chemistry, Vol.97, 349-355, May, 2021

DOI10.1016/j.jiec.2021.02.020 Export Citation

Microvalve-controlled miniaturized electrochemical lab-on-a-chip based biosensor for the detection of β-amyloid biomarker

Satish Kasturi, Sri Ramulu Torati, Yunji Eom, and CheolGi Kim^†

Department of Emerging Materials Science, DGIST, Daegu 42988, Republic of Korea

E-mail:

Recently, miniaturized electrochemical biosensors have the advantage of real-time monitoring and labelfree detection of biomarkers. However, controlled manipulation of reagent samples for specific immobilization of biomarkers remains a challenge to attend high-resolution microfluidic biosensor. Here, we designed a microfluidic channel and valves integrated electrochemical biosensor for the detection of β-amyloid (1-42) biomarker, one of the most neurotoxic peptides present in the cerebrospinal fluid, and a possible marker for the detection of Alzheimer’s disease. The formation of the antibody-antigen complexes on gold microelectrode integrated inside the fluidic chambers was presented in the developed biosensor, and all the measurements are carefully controlled using pneumatic valves integrated with the device. The demonstrated microfluidic electrochemical biosensor exhibits a linear response towards various β-amyloid antigen concentrations from 2.2 pM to 22 μM, with an enhanced detection limit of 1.62 pM. The developed technology offers an advantage of minimal reagent sample and suitable environment for miniaturized and sensitive detection of biomarkers, could also be used for the detection of other biomarkers with high efficiency.

Keywords:Microfluidic channel and valve;Biosensor;Lab-on-a-chip;Alzheimer’s disease;β-Amyloid (1-42) biomarker

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[1] Yu Y, Sun X, Tang D, Li C, Zhang L, Nie D, Yin X, Shi G, Biosens. Bioelectron., 68, 115 (2015)

[2] Song C, Deng P, Que L, Nanomed. Nanotechnol. Biol. Med., 14(6), 1845 (2018)

[3] Kaushik A, Shah P, Vabbina PK, Jayant RD, Tiwari S, Vashist A, Yndart A, Nair M, Analyt. Methods, 8(31), 6115 (2016)

[4] Kim K, Kim MJ, Kim SY, Park S, Park CB, Nat. Commun., 11(1), 1 (2020)

[5] Wu CC, Ku BC, Ko CH, Chiu CC, Wang GJ, Yang YH, Wu SJ, Electrochim. Acta, 134, 249 (2014)

[6] Rama EC, Gonzalez-Garcia MB, Costa-Garcia A, Sens. Actuators B-Chem., 201, 567 (2014)

[7] Olivieri G, Brack C, Muller-Spahn F, Stahelin HB, Herrmann M, Renard P, Brockhaus M, Hock C, J. Neurochem., 74(1), 231 (2000)

[8] Brinet D, Kaffy J, Oukacine F, Glumm S, Ongeri S, Taverna M, Electrophoresis, 335(23), 3302 (2014)

[9] Kotarek JA, Johnson KC, Moss MA, Analyt. Biochem., 378(1), 15 (2008)

[10] Cairo CW, Strzelec A, Murphy RM, Kiessling LL, Biochemistry, 41(27), 8620 (2002)

[11] Chou IH, Benford M, Beier HT, Cote GL, Wang M, Jing N, Kameoka J, Good TA, Nano Lett., 8(6), 1729 (2008)

[12] Lv G, Shen Y, Zheng W, Yang J, Li C, Lin J, Adv. Ther., 2(9), 190005 (2019)

[13] Lien TTN, Takamura Y, Tamiya E, Mun’delanji CV, Anal. Chim. Acta, 892, 69 (2015)

[14] Ramulu TS, Venu R, Sinha B, Lim B, Jeon SJ, Yoon SS, Kim CG, Biosens. Bioelectron., 40(1), 258 (2013)

[15] Alam MK, Rahman MM, Elzwawy A, Torati SR, Islam MS, Todo M, Asiri AM, Kim D, Kim C, Electrochim. Acta, 241, 353 (2017)

[16] Zhu Z, Shi L, Feng H, Zhou HS, Bioelectrochemistry, 101, 153 (2015)

[17] Liu J, Wang J, Wang T, Li D, Xi F, Wang J, Wang E, Biosens. Bioelectron., 65, 281 (2015)

[18] Lee G, Lee J, Kim J, Choi HS, Kim J, Lee S, Lee H, Sci. Rep., 7(1), 1 (2017)

[19] Kong FY, Xu-Zhu, Xu MT, Xu JJ, Chen HY, Electrochim. Acta, 56(25), 9386 (2011)

[20] Ben-Yoav H, Dykstra PH, Bentley WE, Ghodssi R, Biosens. Bioelectron., 64, 579 (2015)

[21] Chen IJ, White IM, Biosens. Bioelectron., 26(11), 4375 (2011)

[22] Ali MA, Srivastava S, Solanki PR, Agrawal VV, John R, Malhotra BD, Appl. Phys. Lett., 101(8), 84105 (2012)

[23] Islam K, Jang YC, Chand R, Jha SK, Lee HH, Kim YS, J. Nanosci. Nanotechnol., 11(7), 5657 (2011)

[24] Kim KW, Reddy V, Torati SR, Hu XH, Sandhu A, Kim CG, Lab Chip, 15(3), 696 (2015)

[25] Dolan GJ, Appl. Phys. Lett., 31(5), 337 (1977)

[26] Torati SR, Kasturi KCSB, Lim B, Kim C, Sens. Actuators B-Chem., 243, 64 (2017)

[27] Qian XC, Zhou X, Qu Q, Li L, Yang L, Electrochim. Acta, 313, 235 (2019)

[28] Kasturi S, Eom YJ, Torati SR, Kim CG, J. Ind. Eng. Chem., 93, 186 (2021)

[29] Torati SR, Reddy V, Yoon SS, Kim C, Biosens. Bioelectron., 78, 483 (2016)

[30] Li H, Cao Y, Wu X, Ye Z, Li G, Talanta, 93, 358 (2012)

[31] Liu L, Zhao F, Ma F, Zhang L, Yang S, Xia N, Biosens. Bioelectron., 49, 231 (2013)

[32] Liu L, He Q, Zhao F, Xia N, Liu H, Li S, Liu R, Zhang H, Biosens. Bioelectron., 51, 208 (2014)

[33] Xia N, Wang X, Zhou B, Wu Y, Mao W, Liu L, ACS Appl. Mater. Interfaces, 8(30), 19303 (2016)