GOx 종이 디스크 전극을 사용한 땀 기반 글루코스 센서

백승엽; 박수영; Seung-Yeop Baek; Soo-Young Park

Polymer(Korea), Vol.46, No.3, 342-347, May, 2022

DOI10.7317/pk.2022.46.3.342 Export Citation

GOx 종이 디스크 전극을 사용한 땀 기반 글루코스 센서

Sweat-based Glucose Biosensor Using a Disc Paper Immobilized with Glucose Oxidase

백승엽, 박수영^†

경북대학교 응용화학공학부 고분자공학전공

Seung-Yeop Baek, and Soo-Young Park^†

Department of Polymer Science & Engineering, Polymeric Nanomaterials Laboratory, School of Applied Chemical Engineering, Kyungpook University, 80 Daehak-ro, Buk-gu, Daegu 41566, Korea

E-mail:

초록

본 연구에서는 상업용으로 판매하는 DS-710 전극(Metrohm DropSens, Spain) 위에 산화 글루코스 효소 (glucose oxidase, 2.25 U disc-1) 를 고정화한 종이 디스크를 덮어 일회용 비침습 글루코스 센서를 제조하였다. 제조 한 종이 글루코스 센서는 전기화학적 chronoamperometric(CA) 방법으로 약 20 μL의 땀에 포함된 글루코스의 양을 -0.155 V에서 선형구간 0.0-4.0 mM과 민감도 0.01 mM로 측정할 수 있었다. 따라서 본 연구에서 개발한 종이 글루 코스 센서는 가격 경쟁력이 있고, 다른 효소에도 적용할 수 있는 확장력이 있는 비침습 바이오 센서이다.

A non-invasive glucose biosensor based on a paper disc immobilized with glucose oxidase (2.25 U disc-1) was developed to be used as a disposable patch on the commercially-available electrode (DS-710, Metrohm DropSens, Spain). The developed glucose biosensor system showed 0.01 mM of limit of detection in the linear range of 0.0 to 4.0 mM when the 20 μL sweat was used to measure a current at applied -0.155 V with a chronoamperometric (CA) method. Thus, this cost-effective and disposable glucose biosensor system can be expandable to other enzyme systems.

Keywords:glucose;non-invasive;biosensor;sweat;disposable

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[2] Amos AF, McCarty DJ, Zimme P, Diabetic Med., 14, S7 (1997)

[3] Tierney MJ, Kim HL, Burns MD, Tamada JA, Potts RO, Electroanalysis, 12, 666 (2000)

[4] Vashist SK, Anal. Chim. Acta, 750, 16 (2012)

[5] Oliver NS, Toumazou C, Cass AEG, Johnston DG, Diabet. Med., 26, 197 (2009)

[6] Lipinska W, Siuzdak K, Karczewski J, Dolega A, Grochowska K, Sens. Actuators B-Chem., 330, 129409 (2021)

[7] Slaughter G, Kulkarni T, Energies, 12, 825 (2019)

[8] Bollella P, Sharma S, Cass AEG, Antiochia R, Electroanalysis, 31, 374 (2019)

[9] Hossain MF, Park JY, Sci. Rep., 6, 21009 (2016)

[10] Karpova EV, Shcherbacheva EV, Galushin AA, Vokhmyanina DV, Karyakina EE, Karyakin AA, Anal. Chem., 91, 3778 (2019)

[11] Bollella P, Gorton L, Ludwig R, Antiochia RA, Sensors, 17, 1912 (2017)

[12] Sabu C, Henna TK, Raphey VR, Nivitha KP, Pramod K, Biosens. Bioelectron., 141, 111201 (2019)

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[14] Zhao WA, Van Den Berg A, Lab Chip, 8, 1988 (2008)

[15] Hussain S, Park SY, ACS Sens., 5, 3988 (2020)

[16] Chi Q, Dong S, Anal. Chim. Acta, 310, 429 (1995)

[17] Karyakin AA, Karyakina EE, Gorton L, Electrochem. Commun., 1, 78 (1999)