Journal of Industrial and Engineering Chemistry, Vol.12, No.5, 745-748, September, 2006
Productive Encystment of Haematococcus pluvialis by Controlling a Specific Irradiation Rate in a Photoautotrophic Induction System for Astaxanthin Production
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A productive photoautotrophic induction system was established for the production of antioxidative astaxanthin by the green microalga Haematococcus pluvialis. During photoautotrophic induction, N-deprived Haematococcus cells were sensitive to the specific irradiation rate (I) on transformation to astaxanthin-rich cyst cells. Compared with the CO2 condition, the optimum specific irradiation rate (Iopt) was confined within narrow limits between light limitation and inhibition. Maximal astaxanthin synthesis in H. pluvialis was promoted under a 5 % CO2 environment and unsynchronized illumination with a specific irradiation rate of 80 μmol photon/gㆍs, yielding an accumulation rate of 4.3 mg/gㆍday. This result indicates that a favorable CO2 concentration and controlled specific irradiation rate leads to the productive encystment of H. pluvialis and enhanced astaxanthin synthesis.
Keywords:astaxanthin;Haematococcus pluvialis;specific irradiation rate;CO2;photoautotrophic induction
- Kobayashi M, Kakizono T, Nagai S, J. Ferment. Bioeng., 71, 335 (1991)
- Margalith PZ, Appl. Microbiol. Biotechnol., 51(4), 431 (1999)
- Boussiba S, Physiol. Plant, 108, 111 (2000)
- Fabregas J, Dominguez A, Maseda A, Otero A, Appl. Microbiol. Biotechnol., 61(5-6), 545 (2003)
- Kobayashi M, Kakizono T, Nagai S, Appl. Environ. Microbiol., 59, 867 (1993)
- Kobayashi M, Kurimura Y, Tsuji Y, Biotechnol. Lett., 19(6), 507 (1997)
- Sarada R, Tripathi U, Ravishankar GA, Process Biochem., 37, 623 (2002)
- Fabregas J, Dominguez A, Alvarez DG, Lamela T, Otero A, Biotechnol. Lett., 20(6), 623 (1998)
- Fabregas J, Otero A, Maseda A, Dominguez A, J. Biotechnol., 89, 65 (2001)
- Hata N, Ogbonna JC, Hasegawa Y, Taroda H, Tanaka H, J. Appl. Phycol., 13, 395 (2001)
- Kakizono T, Kobayashi M, Nagai S, J. Ferment. Bioeng., 74, 403 (1992)
- Orosa M, Franqueira D, Cid A, Abalde J, Biotechnol. Lett., 23(5), 373 (2001)
- Orosa M, Franqueira D, Cid A, Abalde J, Bioresour. Technol., 96(3), 373 (2005)
- Dominguez-Bocanegra AR, Legarreta IG, Jeronimo FM, Campocosio AT, Bioresour. Technol., 92(2), 209 (2004)
- Kang CD, Lee JS, Park TH, Sim SJ, Appl. Microbiol. Biotechnol., 68(2), 237 (2005)
- Yuan JP, Chen F, J. Agric. Food Chem., 46, 3371 (1998)
- Yuan JP, Chen F, Food Chem., 68, 443 (2000)
- Row KH, Polyakova Y, Lee SK, J. Ind. Eng. Chem., 9(5), 473 (2003)
- Kaplan A, Reinhold L, Annu. Rev. Plant Physiol. Plant Mol. Biol., 50, 539 (1999)
- Melis A, Trends Plant Sci., 4, 130 (1999)