The purpose of this work was to access the feasibility and stability of nitrogen and organic matters removal from piggery wastewater in Fixed-bed Biofilm Sequencing Batch Reactor (FbSBR) through the changes of hydraulic retention time (HRT) and substrate concentrations. At steady-state, 80 % total inorganic nitrogen was removed at its applied load of 0.16 kg N/m3/d, while total CODCr removal efficiency was 85 % when its load was at 0.8 kg COD/m3/d. The temporary accumulation of nitrite in the effluent is mostly due to limitation of DO in the inner-layer of biofilm. In the cyclic work on day 61, the occurrence of ORP and DO breakpoints mostly matched to the breakpoint-like of nitrogenous compounds profiles, especially, of NOx trendlines. It would be further utilized for online monitoring to determine operational modes during each steady-state period. The CODCr profiles in cyclic work showed that most biodegradable organic compounds were consumed by facultative bacteria in anoxic phase and only non-biodegradable part was remained during rest of cycle time.