화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.33, No.1, 26-32, January, 2009
Export Citation
이프리플라본을 함유한 생분해성 PLGA 미립구의 제조 및 조직공학적 골재생을 위한 영향평가
Preparation and Release Behavior of Ipriflavone-Loaded PLGA Microsphere for Tissue Engineered Bone
소정원, 장지욱, 김순희, 김근아, 최진희, 이종문, 손영숙1, 민병현2, 강길선†
  • 전북대학교 고분자 BIN소재 융합연구센터
  • 1경희대학교 근골격계 바이오 장기센터
  • 2아주대학교 세포치료센터
Jung Won So, Ji Wook Jang, Soon Hee Kim, Geun Ah Kim, Jin Hee Choi, John M. Rhee, Young Suk Son1, Byoung-Hyun Min2, and Gilson Khang†
  • Polymer BIN Fusion Research Center, Chonbuk National University, 664-14, Dukjin Dong 1Ga, Dukjin Ku, Jeonju 561-756, Korea
  • 1Musculoskeletal Bioorgan Center, Kyunghee University, Global campus,Seocheon Dong, Giheung gu, Yongin 446-701, Korea
  • 2Cell Theraphy Center, School of Medicine, Ajou University, Suwon 442-721, Korea
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초록
골 결손 치료를 위해 생분해성 고분자인 PLGA에 골다공증 치료제인 이프리플라본(IP)을 함유한 미립구를 O/W 유화 용매 증발법으로 제조하였으며, 생체외 방출실험에서 IP 방출량은 HPLC로 분석하였다. SEM을 이용하여 미립구에 부착된 세포의 거동을 확인하였으며, IP가 세포에 미치는 독성평가는 CCK-8 분석방법을 이용하여 측정하였다. 골 형성 지표인 ALP 활성도를 측정하였으며, 배양된 BMSCs의 골세포로의 표현형을 확인하기 위하여 RT-PCR을 수행하였다. 방출결과에서 IP 방출은 거의 40일 이상으로 지속적이었으며, IP를 함유한 미립구에서의 세포의 부착, 성장 등이 잘 이루어짐을 확인하였고, ALP 활성도 및 RT-PCR 분석결과에서도 단독의 PLGA 미립구보다 IP를 함유한 미립구에서의 값이 더 높은 것을 확인할 수 있었다. 본 실험결과를 바탕으로 향후 서방성 제제화에 있어서 IP/PLGA 미립구를 응용하게 되면 국소지향성 골분화 주사용 지지체로서 중요한 역할을 할 것으로 보인다.
The aim of this research was to prepare microparticulate systems based on poly(lactideco- glycolide)(PLGA) for the local release of ipriflavone in order to reduce bone loss. We developed the IP loaded PLGA microspheres using relatively simple oil-in-water(O/W) solvent evaporation method. HPLC was used to perform the in vitro release test of IP and morphology of cell attached on the microspheres was investigated using SEM. Cytotoxicity was assayed by cell counting kit-8 (CCK-8) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed osteocalcin, ALP, and Type I collagen mRNA expression. The release of IP in vitro was more prolonged over 42 days and IP/PLGA microspheres showed the improvement on the cell proliferation, ALP activity and RT-PCR comparing with control (only PLGA). This initial research will be used to direct future work involved in developing this composite injectable bone tissue engineering system.
Keywords:ipriflavone;PLGA;microsphere;osteogenesis;BMSCs.
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