키토산/폴리감마글루탐산 나노입자의 제조 및 중금속 제거에의 응용

성익경; 송재용; 김범수; Ik-Kyoung Sung; Jae Yong Song; Beom Soo Kim

Korean Chemical Engineering Research, Vol.49, No.4, 475-479, August, 2011

Export Citation

키토산/폴리감마글루탐산 나노입자의 제조 및 중금속 제거에의 응용

Preparation of Chitosan/Poly-γ-glutamic Acid Nanoparticles and Their Application to Removal of Heavy Metals

성익경, 송재용, 김범수 ^†

충북대학교 화학공학과, 361-763 충북 청주시 흥덕구 성봉로 410

Ik-Kyoung Sung, Jae Yong Song, and Beom Soo Kim^†

Department of Chemical Engineering, College of Engineering, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju-si, Chungbuk 361-763, Korea

E-mail:

초록

키토산은 천연고분자 물질로 다양한 물리화학적(다중양이온, 반응성 수산화기와 아미노기 그룹), 생물학적(생리활성, 생체적합성, 생분해성) 특성을 가지고 있다. 본 연구에서는 겔형성제로 폴리감마글루탐산을 이용하여 키토산 나노입자를 제조하였다. 나노입자는 폴리감마글루탐산의 카르복실기(-COO^(-))와 키토산의 아미노기(-NH3+)사이의 이온 상호작 용에 의해 형성되었다. 키토산(0.1~1 g)을 100 ml 아세트산 용액(1% v/v)에 첨가한 후 상온에서 충분히 용해되도록 하룻밤 동안 교반하였다. 폴리감마글루탐산(0.1 g)은 상온에서 90 ml 증류수에 용해시켰다. 교반되고 있는 폴리감마글루탐산 용액에 키토산 용액을 주사바늘을 통해 상온에서 적가하였다. 입자의 평균 크기는 80~300 nm 범위에서 형성되었다. 키토산/폴리감마글루탐산 나노입자는 중금속 이온들(Cd2+, Pb2+, Zn2+, Cu2+, Ni2+)의 제거를 위해 콜로이드 상태의 흡착 물질로 사용되었다. 나노입자의 중금속 제거 능력은 Cu2+ > Pb2+ > Cd2+ > Ni2+ > Zn2+의 결과를 보였다.

Chitosan is a natural polymer that has many physicochemical(polycationic, reactive OH and NH2 groups) and biological(bioactive, biocompatible, and biodegradable) properties. In this study, chitosan nanoparticles were prepared using poly-γ-glutamic acid(γ-PGA) as gelling agent. Nanoparticles were formed by ionic interaction between carboxylic groups in γ-PGA and amino groups in chitosan. Chitosan(0.1~1 g) was dissolved in 100 ml of acetic acid (1% v/v) at room temperature and stirred overnight to ensure a complete solubility. An amount of 0.1 g of γ-PGA was dissolved in 90 ml of distilled water at room temperature. Chitosan solution was dropped through needle into beaker containing γ-PGA solution under gentle stirring at room temperature. The average particle sizes were in the range of 80~300 nm. The prepared chitosan/γ-PGA nanoparticles were used to examine their removal of several heavy metal ions(Cd2+, Pb2+, Zn2+, Cu2+ and Ni2+) as adsorbents in aqueous solution. The heavy metal removal capacity of the nanoparticles was in the order of Cu2+ > Pb2+ > Cd2+ > Ni2+ > Zn2+.

Keywords:Nanoparticles;Chitosan;Poly-γ-glutamic Acid;Heavy Metal Removal

[References]

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Akkaya G, Uzun I, Guzel F, Desalination., 9, 1115 (2009)
Guibal E, Saucedo I, Jansson-Charrier M, Delanghe B, Le Cloirec P, Water Sci. Technol., 9, 183 (1994)
Leusch A, Holan Z, Volesky B, J. Chem. Technol. Biotechnol., 10, 279 (1995)
Poo H, Park C, Kwak MS, Choi DY, Hong SP, Lee IH, Lim YT, Choi YK, Bae SR, Uyama H, Kim CJ, Sung MH, Chemistry Biodiversity., 7, 1555 (2010)
Ivanovics G, Erdos L, Z. Immunitatsforsch., 90, 5 (1937)
Sawamura S, J. Coll. Agric. Tokyo., 5, 189 (1913)
Shih IL, Van YT, Bioresour. Technol., 79(3), 207 (2001)
Sung MH, Park C, Kim CJ, Poo H, Soda K, Ashiuchi M, Chemical Record., 5, 352 (2005)
Grenha A, Seijo B, Serra C, Remunan-Lopez C, Biomacromolecules, 8(7), 2072 (2007)
Kawashima Y, Adv. Drug Deliv. Rev., 47, 1 (2001)
Mao HQ, Roy K, Troung-Le VL, Janes KA, Lin KY, Wang Y, August JT, Leong KW, J. Control. Rel., 70, 399 (2001)
Qi L, Xu Z, Colloid Surf. A: Physicochem. Eng. Aspects., 251, 183 (2004)
Chang YC, Chen DH, J. Colloid Interface Sci., 283(2), 446 (2005)
Seo WS, Kim TT, Sung JS, Song KC, Korean Chem. Eng. Res., 42(1), 78 (2004)
Kim BS, Song JY, in: Hou CT, Shaw JF (Ed.), Biocatalysis and Agricultural Biotechnology, CRC Press, 399 (2009)
Kang MK, Kim JC, Polym.(Korea), 34(1), 79 (2010)
Brady JM, Tobin JM, Enzyme Microb. Technol., 17(9), 791 (1995)
Bhattacharyya KG, Gupta SS, Colloid Surf. A: Physicochem. Eng. Aspects., 277, 191 (2006)
Singh SP, Ma LQ, Hendry MJ, J. Hazard. Mater., 36, 654 (2006)
Srinivasa RP, Vijaya Y, Boddu VM, Krishnaiah A, Biores. Technol., 100, 194 (2009)

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[1] Rinaudo M, Prog. Polym. Sci., 31, 603 (2006)

[2] Ieva E, Trapani A, Cioffi N, Ditaranto N, Monopoli A, Sabbatini L, Anal. Bioanal. Chem., 9, 207 (2009)

[3] Kataoka T, Yoshida H, Chem. Eng. J., 8, 107 (1988)

[4] Akkaya G, Uzun I, Guzel F, Desalination., 9, 1115 (2009)

[5] Guibal E, Saucedo I, Jansson-Charrier M, Delanghe B, Le Cloirec P, Water Sci. Technol., 9, 183 (1994)

[6] Leusch A, Holan Z, Volesky B, J. Chem. Technol. Biotechnol., 10, 279 (1995)

[7] Poo H, Park C, Kwak MS, Choi DY, Hong SP, Lee IH, Lim YT, Choi YK, Bae SR, Uyama H, Kim CJ, Sung MH, Chemistry Biodiversity., 7, 1555 (2010)

[8] Ivanovics G, Erdos L, Z. Immunitatsforsch., 90, 5 (1937)

[9] Sawamura S, J. Coll. Agric. Tokyo., 5, 189 (1913)

[10] Shih IL, Van YT, Bioresour. Technol., 79(3), 207 (2001)

[11] Sung MH, Park C, Kim CJ, Poo H, Soda K, Ashiuchi M, Chemical Record., 5, 352 (2005)

[12] Grenha A, Seijo B, Serra C, Remunan-Lopez C, Biomacromolecules, 8(7), 2072 (2007)

[13] Kawashima Y, Adv. Drug Deliv. Rev., 47, 1 (2001)

[14] Mao HQ, Roy K, Troung-Le VL, Janes KA, Lin KY, Wang Y, August JT, Leong KW, J. Control. Rel., 70, 399 (2001)

[15] Qi L, Xu Z, Colloid Surf. A: Physicochem. Eng. Aspects., 251, 183 (2004)

[16] Chang YC, Chen DH, J. Colloid Interface Sci., 283(2), 446 (2005)

[17] Seo WS, Kim TT, Sung JS, Song KC, Korean Chem. Eng. Res., 42(1), 78 (2004)

[18] Kim BS, Song JY, in: Hou CT, Shaw JF (Ed.), Biocatalysis and Agricultural Biotechnology, CRC Press, 399 (2009)

[19] Kang MK, Kim JC, Polym.(Korea), 34(1), 79 (2010)

[20] Brady JM, Tobin JM, Enzyme Microb. Technol., 17(9), 791 (1995)

[21] Bhattacharyya KG, Gupta SS, Colloid Surf. A: Physicochem. Eng. Aspects., 277, 191 (2006)

[22] Singh SP, Ma LQ, Hendry MJ, J. Hazard. Mater., 36, 654 (2006)

[23] Srinivasa RP, Vijaya Y, Boddu VM, Krishnaiah A, Biores. Technol., 100, 194 (2009)