Applied Chemistry for Engineering, Vol.23, No.2, 164-168, April, 2012
유기산 및 효소적 전처리를이용한다시마에서바이오에탄올 생산
Organic Acid and Enzyme Pretreatment of Laminaria japonica for Bio-ethanol Production
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초록
본 연구는 갈조류인 다시마의 당 성분 함량에 대한 분석과 효소 및 유기산 가수분해물을 이용한 생물학적 바이오에탄올 생산에 대해 연구하였다. HPLC를 이용한 당 성분 분석 결과 alginate가 total sugar의 65.99%로 가장 많은 것으로 확인되었으며, laminaran과 mannitol이 각각 6.24, 27.77%로 나타났다. 1.5% acetic acid를 이용하여 121 ℃, 60 min 동안 가수분해 결과 최대 1.874 g/L의 환원당이 생성되었으며, ascorbic acid의 경우 2.0%에서 최대 4.291 g/L의 환원당이 생성되는 것으로 나타났다. Alginate lyase와 laminarinase와 같은 효소를 이용한 가수분해에서 환원당 생성량은 최대 2.219 g/L였다. 다시마 가수분해물을 이용한 에탄올 발효 결과 유기산을 처리했을 때에는 에탄올 생산량이 오히려 감소하는 것으로 나타났으며, alginate lyase와 laminarinase를 혼합처리 했을 때 에탄올 생산량이 1.26 g/L로 가장 높았다.
We investigated for the production of biological bio-ethanol from Laminaria japonica using the hydrolysis reaction of enzymes and organic acids and the polysaccharide content was also analyzed. The composition of the polysaccharide was characterized as 65.99% alginate, 6.24% laminaran and 27.77% mannitol. The optimum concentration for reducing the sugar conversion
by Laminaria japonica was found to be 1.874 g/L at an acetic acid concentration of 1.5%, 121 ℃ for 60 min, and for an ascorbic acid of 2.0%, 4.291 g/L was produced in the same condition. The enzyme hydrolysis such as alginate lyase and laminarinase contained the maximum 2.219 g/L reducing sugar. In the result of ethanol fermentation using hydrolysate of Laminaria japonica, the organic acid treatment showed a high of reducing sugar yield, but decreased the ethanol yield,
and then the maximum ethanol production obtained was 1.26 g/L using the mixed treated of enzyme.
- Park JI, Woo HC, Lee JH, Korean Chem. Eng. Res., 46(5), 833 (2008)
- Lee SM, Kim JH, Cho HY, Joo H, Lee JH, J. Korean Ind. Eng. Chem., 20(5), 517 (2009)
- Balat M, Balat H, Applied Energy., 86, 2273 (2009)
- Tolbert NE, Regulation of atmospheric CO2 and O2 by photosynthetic Carbon Metabolism, ed. J. Preiss, 8, Oxford University Press, Oxford (1994)
- Do JR, Nam YJ, Park JH, Jo JH, J. Kor. Fish. Soc., 30, 428 (1997)
- Hirano A, Ueda R, Hirayama S, Ogushi Y, Energy, 22(2-3), 137 (1997)
- Saha BC, Cotta MA, Enzyme Microb. Technol., 41(4), 528 (2007)
- Hahn-Hagerdal B, Galbe M, Gorwa-Grauslund MF, Liden G, Zacchi G, Trends Biotechnol., 24, 549 (2006)
- Renewable Global Status Report. Renewable energy network for the 21st century (REN21). Washington, DC: Worldwatch Institute Paris, REN21 Secretariat (2009)
- Kim JH, Byun DS, Godber JS, Choi JS, Choi WC, Kim HR, Appl. Microbiol. Biotechnol., 63(5), 553 (2004)
- Kang HI, Ko MS, Kim HJ, Kim SW, Bae TJ, J.Kor. Fish. Soc., 29, 716 (1996)
- Lee SA, Kim JU, Jung JG, Kim IH, Lee SH, Kim SJ, Lee JH, Kor. J. Biotechnol. Bioeng., 21, 389 (2006)
- Park YH, Bull. Korean Fish. Soc., 2, 71 (1969)
- Lee SM, Lee JH, Appl. Chem. Eng., 21(2), 154 (2010)
- Choi DB, Sim HS, Piao YL, Ying W, Cho H, J. Ind. Eng. Chem., 15(1), 12 (2009)