화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.37, No.6, 669-676, November, 2013
Export Citation
In vivo 상에서 탈미네랄화된 골분이 함유된 PLGA 지지체를 이용한 추간판 디스크 재생
Regeneration of Intervertebral Disc Using Poly(lactic-co-glycolic acid) Scaffolds Included Demineralized Bone Particle In Vivo
장지은, 김혜윤, 송정은, 이동원, 권순용1, 정진화1, †, 강길선†
  • 전북대학교 BIN 융합공학과, 고분자나노공학과
  • 1가톨릭대학교 의과대학 여의도성모병원 정형외과
Ji Eun Jang, Hye Yoon Kim, Jeong Eun Song, Dongwon Lee, Soon Yong Kwon1, Jin Wha Chung1, †, and Gilson Khang†
  • Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ., 567 Baekje-daero, Jeonju 561-756, Korea
  • 1Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
E-mail:,
초록
탈미네랄화된 골분(demineralized bone particle, DBP)은 연골 형성의 유도인자로 사용되기 때문에 조직공학에서 널리 사용되는 생체재료이다. 본 연구에서는 in vivo 환경에서 디스크 재생 효과를 연구하기 위해 DBP를 poly(lactic-co-glycolic acid) (PLGA)에 첨가하여 다공성 지지체를 제조하였다. 디스크의 섬유륜 조직을 반으로 절개한 후, 수핵 조직을 제거하여 디스크 결손을 유발시켰다. 빈 공간에 PLGA, DBP/PLGA 지지체를 이식하여 in vivo 환경에서 조직공학적 디스크 재생을 관찰하였다. 1, 2 및 3개월 후 디스크를 적출하여 글리코스아미노글라이칸 (glycosaminoglycan, sGAG) 및 콜라겐 합성 정도를 측정하였으며 조직학적 평가로 H&E, Safranin-O, Alcian blue 염색과 면역조직학적 평가로 제 I형 콜라겐, 제 II형 콜라겐 염색을 수행하였다. 그 결과 DBP/PLGA 지지체에서 sGAG 및 콜라겐 함량이 높은 것을 확인하였으며 추간판 디스크 재생의 가능성을 확인하였다.
Demineralized bone particle (DBP) is a biomaterial used widely in the field of tissue engineering. In this study, in order to study the effect of DBP/poly(lactic-co-glycolic acid) (PLGA) scaffold on disc regeneration in vivo environment, we prepared the porous DBP/PLGA hybrid scaffold. Disc defect was induced by removing the nucleus pulposus tissue after incision the annulus fibrosus tissue in half and scaffolds were transplanted. After 1, 2 and 3 months later, the extracted discs were confirmed by collagen synthesis and glycosaminoglycan (sGAG). We conducted histology (H&E, Safranin-O, Alcian blue, Type I Collagen, Type II Collagen). From the results, it was confirmed that collagen and sGAG content were high in DBP/PLGA scaffold, and the regeneration of intervertebral disc was possible.
Keywords:demineralized bone particle;poly(lactic-co-glycolic acid);scaffold;disc regeneration.
  1. Endres M, Abbushi A, Thomale UW, Cabraja M, Kroppenstedt SN, Morawietz L, Casalis PA, Zenclussen ML, Lemke AJ, Horn P, Kaps C, Woiciechowsky C, Biomaterials., 31, 5836 (2010)
  2. Shankar H, Scarlett JA, Abram SE, Tech. Reg. Anesth.Pain. Manag., 13, 67 (2009)
  3. Hsieh AH, Twomey JD, J. Biomech., 43, 137 (2010)
  4. Adams MA, Dolan P, McNally DS, Matrix Biology., 28, 384 (2009)
  5. Bibby SRS, Jones DA, Lee RB, Yu J, Urban JPG, Joint Bone Spine., 68, 537 (2001)
  6. Endres M, Abbushi A, Thomale UW, Cabraja M, Kroppenstedt SN, Morawietz L, Casalis PA, Zenclussen ML, Lemke AJ, Horn P, Kaps C, Woiciechowsky C, Biomaterials., 31, 5836 (2010)
  7. Lee SK, Hong HK, Kim SJ, Kim YK, Song YS, Ha Y, Lee D, Khang G, Polym.(Korea), 34(5), 398 (2010)
  8. Zhou GQ, Yang F, Leung VVL, Cheung KMC, Curr.Orthopaed., 22, 267 (2008)
  9. Adams MA, Roughley PJ, Spine(Phila Pa 1976)., 31, 2151 (2006)
  10. Jin L, Feng G, Reames DL, Shimer AL, Shen FH, Li X, Spine J., 13, 235 (2013)
  11. Gruber HE, Leslie K, Ingram J, Norton HJ, Hanley EN, Spine J., 4, 44 (2004)
  12. Melrose J, Smith S, Ghosh P, Taylor TKF, Cells Tissues Organs., 168, 137 (2001)
  13. Shao X, Hunter CJ, J. Biomed. Mater. Res. Part A., 82A, 701 (2007)
  14. Hong HK, Lee SK, Song YS, Kim DS, Eom S, Kim HE, Lee D, Khang G, Polym.(Korea), 34(3), 282 (2010)
  15. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
  16. Woo Y, Na K, Inter. J. Tissue Regen., 3, 63 (2012)
  17. Gu BK, Kim MS, Park SJ, Kim CH, Inter. J. Tissue Regen., 2, 83 (2011)
  18. Yang DH, Park HN, Lee JB, Heo DN, Bae MS, Kwon IK, Inter. J. Tissue Regen., 2, 119 (2011)
  19. Kolse D, Delplace C, Siepmann J, Int. J. Pharm., 404, 75 (2011)
  20. Carballido AF, Pastoriza P, Barcia E, Montejo C, Negro S, Int. J. Pharm., 352, 50 (2008)
  21. Jo EH, Kim SJ, Cho SJ, Lee GY, Kim OY, Lee EY, Cho WH, Lee DW, Khang G, Polym.(Korea), 35(4), 289 (2011)
  22. Mi FL, Lin YM, Wu YB, Shyu SS, Tsai YH, Biomaterials., 23, 3257 (2002)
  23. Bae HW, Zhao L, Kanim LEA, Pamela PBS, Delamarter RB, Dawson EG, Spine., 31, 1299 (2006)
  24. Mizuno S, Glowacki J, Cells Tissues Organs., 180, 151 (2005)
  25. Yoon SJ, Kim SK, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)
  26. Kim AR, Kim HM, Lee JK, Lee JH, Song JE, Yoon KH, Lee D, Khang G, Polym.(Korea), 36(6), 768 (2012)
  27. Lee YM, Shim CR, Lee Y, Kim HN, Jo SA, Song JE, Lee D, Khang G, Polym.(Korea), 36(6), 789 (2012)
  28. Ko YK, Jeong JS, Kim SH, Lim JY, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 32(2), 109 (2008)
  29. Urist MR, Science., 150, 893 (1965)
  30. Iatridis JC, Nicoll SB, Michalek AJ, Walter BA, Gupta, MS, Spine. J., 13, 243 (2013)
  31. Kim HE, Kim HN, Yu H, Song JE, Jeoung SY, Kim Y, Lee D, Khang G, Macromol. Res., 20(10), 1044 (2012)
  32. Hegewald AA, Zouhair S, Endres M, Mario , Cabraja, Woiciechowsky C, Thome C, Kaps C, Tissue and Cell., 45, 68 (2013)
  33. Kang YH, J. Korean Spine Surg., 8, 264 (2001)
  34. Whatley BR, Wen X, Mat. Sci. Eng. C., 32, 61 (2012)
  35. Moon SH, J. Korean Spine Surg., 14, 120 (2007)
  36. Lee SK, Hong HK, Kim SJ, Kim YK, Song YS, Ha Y, Lee D, Khang G, Polym.(Korea), 34(5), 398 (2010)
  37. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)