화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.39, No.1, 144-150, January, 2015
Export Citation
PLGA 미립구가 함유된 젤란검 스폰지를 이용한 추간판 조직 재생
Regeneration of Intervertebral Disc Using Gellan Sponge Loading PLGA Microspheres
박현우, 김혜윤1, 권순용2, 강길선1, 김용식3, †
  • 단국대학교 의과대학 정형외과
  • 1전북대학교 BIN융합공학과.고분자나노공학과
  • 2가톨릭의과대학교 여의도성모병원 정형외과
  • 3가톨릭의과대학교 서울성모병원 정형외과
Hyunwoo Park, Hye Yun Kim1, Soon Yong Kwon2, Gilson Khang1, and Yong-Sik Kim3, †
  • Department of Orthopedic Surgery, Dankook University Hospital, 201 Manghyangro, Dongnam-gu, Cheonan-si, Chungcheongnam-do 330-715, Korea
  • 1Department of BIN Fusion Technology, Department of Polymer-Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonju 561-756, Korea
  • 2Department of Orthopedic Surgery, Yeouido St.Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-896, Korea
  • 3Department of Orthopedic Surgery, Seoul St.Mary’s Hospital, Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, Korea
E-mail:
초록
젤란검은 천연재료로써 내열성, 내산성, 내효소성 등이 우수하여 용도가 광범위하나 기계적 강도가 약하다는 단점이 있다. 따라서 본 연구에서는 기계적 성질을 개선하고자 젤란검 스폰지에 PLGA 미립구를 혼합하였다. PLGA 미립구의 다양한 함량의 젤란검 스폰지는 기계적 강도를 알아보고자 압축강도를 측정하였고, MTT 분석, SEM, 생체활성조직학적 평가 및 RT-PCR을 통해 세포의 증식 및 ECM 분비 효과를 확인하였다. 그 결과, PLGA 미립구가 50% 함유된 젤란검 지지체에서 섬유륜세포의 꾸준한 증식과 세포외기질 분비가 우수한 것을 확인할 수 있었다. 이 연구를 통하여 PLGA 미립구가 함유된 젤란검이 디스크조직 재건을 위한 지지체로서 적합함을 알 수 있었으며, 젤란검의 다양한 응용가능성을 제시하였다.
Gellan gum as a natural polysaccharide has good heat resistance, acid resistance and enzymes resistance. However, one of the drawbacks of gellan gum might be the lower mechanical strength. In this work, gellan gum scaffolds were mixed with poly(lactic-co-glycolic acid) (PLGA) microsphere in order to improve mechanical properties. The gellan gum scaffolds with various contents of PLGA microsphere were prepared for the regeneration of disc tissues. To evaluate the mechanical strength of hybrid structure of gellan gum and PLGA microsphere, compression strength of the fabricated scaffolds was measured. MTT analysis, SEM observation, histological evaluation and RT-PCR were performed to confirm the effect on the cell growth and extracellular matrix secretion. As a result, it showed the best cell proliferation and extracellular matrix secretion in gellan gum sponge containing 50% PLGA microspheres. In conclusion, this study confirmed that the hybrid structure of gellan gum and PLGA microspheres was found suitable in regeneration of the intervertebral disc.
Keywords:intervertebral disc;gellan gum;PLGA microsphere;cell proliferation;tissue engineering disc regeneration.
  1. Wollbold J, Huber R, Pohlers D, Koczan D, Guthke R, Kinne RW, Gausmann U, Bmc. Syst. Biol., 3, 77 (2009)
  2. Reza AT, Nicoll SB, Acta Biomater., 6, 179 (2010)
  3. Williamson AK, Chen AC, Sah RL, J. Orthopaed. Res., 19, 1113 (2001)
  4. Hayes AJ, Isaacs MD, Hughes C, Caterson B, Ralphs JR, Eur. Cells Mater., 22, 226 (2011)
  5. Jacobs NT, Smith LJ, Han WM, Morelli J, Yoder JH, Elliott DM, J. Mech. Behav. Biomed., 4, 1611 (2011)
  6. Wang D, Vo NV, Sowa GA, Hartman RA, Ngo K, Choe SR, Witt WT, Dong Q, Lee JY, Niedernhofer LJ, Kang JD, Spine J., 11, 139 (2011)
  7. Borges AC, Eyholzer C, Duc F, Bourban PE, Tingaut P, Zimmermann T, Pioletti DP, Manson JA, Acta Biomater., 7, 3412 (2011)
  8. Cheng YH, Yang SH, Su WY, Chen YC, Yang KC, Cheng WT, Wu SC, Lin FH, Tissue Eng., Part A, 16, 695 (2010)
  9. Gruber HE, Leslie K, Ingram J, Norton HJ, Hanley EN, Spine J., 4, 44 (2004)
  10. Yeganegi M, Kandel RA, Santerre JP, Acta Biomater., 6, 3847 (2010)
  11. Pereira DR, Silva-Correia J, Caridade SG, Oliveira JT, Sousa RA, Salgado AJ, Oliveira JM, Mano JF, Sousa N, Reis RL, Tissue Eng., Part C, 17, 961 (2011)
  12. Khang G, Lee S, Kim H, Silva-Correia J, Gomes M, Viegas C, Dias I, Oliveira J, Reis R, J. Tissue Eng. Regen. Med., DOI: 10.1002/term.1625 (2012)
  13. Silva-Correia J, Miranda-Goncalves V, Salgado AJ, Sousa N, Oliveira JM, Reis RM, Reis RL, Tissue Eng., Part A, 18, 1203 (2012)
  14. Matricardi P, Cencetti C, Ria R, Alhaique F, Coviello T, Molecules, 14, 3376 (2009)
  15. Oliveira JT, Santos TC, Martins L, Silva MA, Marques AP, Castro AG, Neves NM, Reis RL, J. Tissue Eng. Regen. Med., 3, 493 (2009)
  16. Oliveira JT, Santos TC, Martins L, Picciochi R, Marques AP, Castro AG, Neves NM, Mano JF, Reis RL, Tissue Eng., Part A, 16, 343 (2010)
  17. Ahuja M, Singh S, Kumar A, Inter. J. Biol. Macromol., 53, 114 (2013)
  18. Chen Q, Zheng Y, Li Y, Zeng Y, Kuang J, Hou S, Li X, Drug Deliv., 19, 194 (2012)
  19. Silva-Correia J, Oliveira JM, Caridade SG, Oliveira JT, Sousa RA, Mano JF, Reis RL, J. Tissue Eng. Regen. Med., 5, 97 (2011)
  20. Jones AC, Arns CH, Hutmacher DW, Milthorpe BK, Sheppard AP, Knackstedt MA, Biomaterials, 30, 1440 (2009)
  21. Rose FRAJ, Oreffo ROC, Biochem. Biophys. Res. Commun., 292(1), 1 (2002)
  22. Kim HE, Kim HN, Yu H, Song JE, Jeoung SY, Kim Y, Lee D, Khang G, Macromol. Res., 20(10), 1044 (2012)
  23. Gu BK, Kim MS, Park SJ, Kim CH, Inter. J. Tissue Regen., 2, 83 (2011)
  24. Woo Y, Na K, Inter. J. Tissue Regen., 3, 63 (2012)
  25. Guanzon JN, Berg JR, Mizuno M, Lee S, Jo Y, Yeo J, Nam B, Kim B, Koh Y, Takebe T, Lee J, Inter. J. Tissue Regen., 4, 7 (2013)
  26. Kim HL, Hong M, Kim SJ, Jo H, Yoo IS, Lee D, Khang G, Polym.(Korea), 35(5), 378 (2011)
  27. Kuettner KE, Clin. Biochem., 25, 155 (1992)
  28. Sha'ban M, Yoon SJ, Ko YK, Ha HJ, Kim SH, So JW, Idrus RB, Khang G, J. Biomater. Sci., Polym. Ed., 19, 1219 (2008)
  29. Huang YX, Ren J, Chen C, Ren TB, Zhou XY, J. Biomater. Appl., 22, 409 (2008)
  30. Lu H, Hoshiba T, Kawazoe N, Chen G, Biomaterials, 32, 2489 (2011)
  31. Liu H, Yao F, Zhou Y, Yao K, Mei D, Cui L, Cao Y, J. Biomater. Appl., 19, 303 (2005)